Introduction

Dengue fever, this mosquito-borne infectious disease, known as “broken bone fever”, is spreading around the world at an unprecedented rate. Its complex morbidity mechanism, especially the lethal antibody-dependent enhancement (ADE), makes rapid and precise differential diagnosis a primary challenge in the clinical and public health fields.

How to effectively distinguish between primary infection and high-risk secondary infection, and how to identify the virus at an early stage of infection? This is not only a difficult problem for doctors, but also a fortress that IVD developers must overcome.

As your professional partner in the field of in vitro diagnosis, Maiyue Biology will take you in-depth today, from the core of the morbidity mechanism of dengue fever to the choice of diagnostic methods, and finally present you with a one-stop core raw material solution that has been strictly verified by the market.

source:network

1、Meet Our Opponent: Dengue Virus

Dengue fever is an acute infectious disease caused by dengue virus, which belongs to Flaviviridae and Flavivirus. It has the following main features:

  • Four major serotypes: DENV-1, DENV-2, DENV-3, DENV-4. Infection with one type confers long-lasting immunity to that type, but only transient cross-protection to the other types.
  • Severe risk: Re-infection with viruses of different serotypes is a key risk factor for severe dengue fever (such as hemorrhagic fever and shock syndrome).

2、Core mechanism: The deadly “Trojan horse” -ADE effect

ADE effect that is, antibody-dependent enhancement effect, is an important mechanism in infectious diseases such as dengue fever, which may aggravate symptoms of infection and even affect the development and clinical application of vaccines.

The core of the pathogenesis of dengue virus lies in its complex game with the host immune system, especially the antibody-dependent Enhancement (ADE) during secondary infection.

  • Initial infection: Once the virus invades, it replicates rapidly in the host, triggering viremia and a series of typical symptoms. Immediately thereafter, the body will initiate defense mechanisms to produce neutralizing antibodies to effectively eliminate the virus, thereby conferring lasting immune protection to the host against that specific serotype.
  • Secondary infection and ADE: When a patient is reinfected with a virus of a different serotype, the IgG antibody produced by the primary infection in the body (non-neutralizing or sub-neutralizing at this time) will bind to the virus like a “Trojan horse” and “invite the wolf into the house” through its Fc segment to help the new virus infect immune cells more efficiently. This results in a dramatic increase in viral load (up to 10-1000 times that of the initial infection) and triggers a “cytokine storm” that can eventually lead to fatal severe disease.

Conclusion: Diagnostic tools that can distinguish primary infection from secondary infection are essential for clinical prognosis.

3、Epidemic Alert: Global Epidemic and China Challenge

Dengue fever is widespread in tropical and subtropical regions of the world. In China, Guangdong, Guangxi, Yunnan and other southern provinces are the traditional high incidence areas, and the epidemic has spread northward in recent years. Accurate and rapid diagnosis is the lifeline to control the spread of the epidemic.

4、Diagnostic “Arsenal”: Comparison of Three Technical Paths

In the face of dengue fever, we have three major detection technologies. Choosing the right diagnostic tool and applying it at the critical moment is undoubtedly the decisive factor to ensure the success of diagnosis.

5、The Cornerstone of Accurate Diagnosis: Selection of Core Raw Materials and Leap Program

Excellent diagnostic reagents are based on high-performance core raw materials. Maiyue Biology provides a complete set of optimized raw material solutions for different stages and needs of dengue fever diagnosis.

5.1 “Pioneer” of Early Diagnosis: Raw Material of NS1 Antigen Detection

Why NS1? NS1 protein is a marker secreted into the blood in large quantities during viral replication, which appears early and has high concentration, and is an irreplaceable target for early diagnosis.

Bio-Mapper NS1 Raw Material Solution: We are committed to providing customers with solid confidence in the development of NS1 detection reagents with high sensitivity, broad spectrum coverage and high specificity.

√Recommended Antibody Pairs (for Sandwich Development):

  •  Broad spectrum combination (Type I + II + III + IV): MC320401/MC320403 (coated) with MC320402 (labeled) is recommended and has been extensively validated to provide balanced coverage of the four major serotypes.
  • I + II + III combination: MC320101 (coated) + MC320102 (labeled)
  • Type IV specific combination: MC320201/MC320301 (coated) + MC320202/MC320302 (labeled)

Quality control (for performance verification and production quality control): We provide high-purity HEK293 cells expressing recombinant NS1 antigen (PE320101-PE320401) covering DENV type I-IV to ensure that your quality control system is perfect and reliable.

5.2 Infection Staging “Defender”: Antibody Test Material (IgM/IgG)

Why choose E protein? Envelope protein (E) is the main target to induce the body to produce antibodies. Using high-quality recombinant E protein as the detection antigen can most truly reflect the antibody level in serum.

Bio-Mapper Antibody Detection Raw Material Solution: We provide high-purity, high-activity recombinant antigens and high-quality capture antibodies to help you develop reagents that can accurately determine the stage of infection and effectively distinguish between primary and secondary infections.

6、The Ultimate Strategy: “triple Inspection”-A Card, Comprehensive Insight

Integrating NS1 antigen, IgM antibody and IgG antibody into one test card is the best strategy for POCT diagnosis at present.

√Clinical value of a card:

  • NS1 (+), IgM (-), IgG (-): very early primary infection (seize the best opportunity for intervention)
  • NS1 (+), IgM (+), IgG (-): primary infection in acute phase
  • NS1 (+/-), IgM (+), IgG (+): secondary infection in the acute phase or in the middle and late stages of primary infection (alert to the risk of severe illness!)
  • NS1 (-), IgM (-), IgG (+): previous infection

The complete set of raw materials provided by Bio-Mapper can perfectly support the development of dengue fever triple inspection products. In addition, Bio-Mapper also provides dengue fever colloidal gold semi-finished products with high sensitivity and specificity, which are clinically proven, helping to shorten the time to market for customers.

Why Choose Bio-Mapper? — — Your Trusted IVD Core Raw Material Partner

In the current epidemic situation, stable, efficient and reliable diagnostic products are the key to success. If you choose Maiyue Biology, you will get:

  1. Excellent performance: All raw materials are subject to strict performance verification, including the verification of intra-batch precision, inter-batch precision, accuracy, linear range, reference interval, reportable interval and other parameters, to ensure high sensitivity, high specificity and small inter-batch difference.
  2. Comprehensive solution: from NS1 to E protein, from antigen to antibody, we provide “one-stop” raw material supply to accelerate your research and development process.
  3. Stable supply: Strong production capacity and inventory guarantee ensure that you have enough “ammunition” during the peak period of market demand.
  4. Professional support: Our experienced technical support team is ready to accompany you from raw material evaluation to product optimization.

It’s time to add more momentum to your dengue fever Diagnostics product line!

Contact us today for free samples of high performance ingredients and one-on-one technical support!

Mailbox: info@bio-mapper.com

Whatsapp:+86 15168500284